Unmet needs in cell isolation
Cell isolation from organ or tissues represents a critical step: not only a high number of living and functional cells needs to be isolated, but also reproducible protocols and results are necessary.
Proper enzymes selection is perhaps the most important determinant for successful isolation.
The gold standard for performing cell isolation is the enzymatic digestion of the target tissue/organ by Collagenases
- Current technologies to produce collagenases are based on the culture of C. Hystolyticum and the subsequent proteins purification thereof. The resulting ‘extractive Collagenase’ are actually blends, containing different percentage ratios of the two collagenase isoforms (class I and class II), plus a number of proteases.
- Variability in composition and residual trypsin-like activity determine unpredictable enzymatic efficiency, low batches consistency and low stability, that can cause drawbacks in the efficiency of cell extraction and low reproducibility and standardization of protocols.
- To date researchers/clinicians face difficulties with “extractive collagenases” and must perform a trial-and-error process to lot selection and to fine-tune the collagenases and protease blend that maximises the cell extraction process, thus increasing the protocols’ costs due to biological and enzymatic material wasted as well as staff/instrumentation involved.
Efforts to improve reproducibility are important: biological samples can often be used only once and time donated by volunteers is precious.
The new face of tissue dissociation enzymes
ABIEL COL G – COL H RECOMBINANT COLLAGENASES
Abiel Recombinant Collagenases represent a next generation collagenases, designed to improve cell isolation reproducibility and standardization, enhancing cell yield and viability.
Abiel has synthesized innovative Recombinant Collagenases class I (COL G) and class II (COL H) by a patented technology in E. Coli strain. The recombinant production process (animal-free) ensures a high level of purity, stability and a remarkable lot-to-lot consistency. The production of each enzymatic component per run avoids instability and allows formulating customized and standardized blends.
Depending on the application, COL G is used in combination with COL H to optimize the isolation protocol and to obtain a higher amount of functional cells. The blending is based on activity determination and not on weight ratios.
Saving cost and time, use Abiel’s collagenases. Ensuring the standardization of your cell isolation protocol
Benefits of Abiel’s enzymes
- Reproducible batches: avoid batch-to-batch variation.
- Consistency: consistent results from each experiment. Time saving–cost saving.
- Pure collagenase (>99%): no contaminating proteases, which reduce over-digestion.
- Reagent Flexibility: control collagenase digestion rate (or aggressiveness) adapted to protocol
- High stability (up to 3 years): Lyophilised product stable at RT or cold storage; no autodegradation by proteases.
- Endotoxin-free: No off-shoot toxic effects on cells.
- Cost saving: thanks to standardized results overtime with less biological and enzymatic material wasted as well as staff/instrumentation involved.
- Abiel‘s Kit package containing COL G and/or COL H and Thermolysin is ready-to-use which allows producing fully reproducible and standardized blends with highly predictable enzymatic efficiency.